全文获取类型
收费全文 | 99965篇 |
免费 | 1031篇 |
国内免费 | 1520篇 |
出版年
2023年 | 86篇 |
2022年 | 114篇 |
2021年 | 351篇 |
2020年 | 270篇 |
2019年 | 305篇 |
2018年 | 12070篇 |
2017年 | 10866篇 |
2016年 | 7718篇 |
2015年 | 1057篇 |
2014年 | 838篇 |
2013年 | 887篇 |
2012年 | 4847篇 |
2011年 | 13288篇 |
2010年 | 12293篇 |
2009年 | 8499篇 |
2008年 | 10128篇 |
2007年 | 11654篇 |
2006年 | 559篇 |
2005年 | 760篇 |
2004年 | 1221篇 |
2003年 | 1227篇 |
2002年 | 1023篇 |
2001年 | 444篇 |
2000年 | 318篇 |
1999年 | 162篇 |
1998年 | 128篇 |
1997年 | 113篇 |
1996年 | 89篇 |
1995年 | 74篇 |
1994年 | 69篇 |
1993年 | 79篇 |
1992年 | 71篇 |
1991年 | 76篇 |
1990年 | 33篇 |
1989年 | 26篇 |
1988年 | 43篇 |
1987年 | 34篇 |
1986年 | 12篇 |
1985年 | 13篇 |
1984年 | 36篇 |
1983年 | 25篇 |
1982年 | 8篇 |
1972年 | 246篇 |
1971年 | 274篇 |
1970年 | 5篇 |
1965年 | 13篇 |
1962年 | 24篇 |
1956年 | 5篇 |
1944年 | 12篇 |
1940年 | 10篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
101.
Torre J. Hovick Brady W. Allred Devan A. McGranahan Michael W. Palmer R. Dwayne Elmore Samuel D. Fuhlendorf 《Biodiversity and Conservation》2016,25(2):345-356
A species distribution combines the resources and climatic tolerances that allow an individual or population to persist. As these conditions change, one mechanism to maintain favorable resources is for an organism to shift its range. Much of the research examining range shifts has focused on dynamic distribution boundaries wheras the role of species breeding habitat or migration strategies on shift tendencies has received less attention. We expand on previous research by using a large suite of avian species (i.e., 277), analyzing observed abundance-weighted average latitudes, and categorizing species by breeding environment and migration strategy. We used the North American Breeding Bird Survey dataset to address two questions: (1) Has the center of observed abundance for individual species shifted latitudinally? (2) Is there a relationship between migration strategy or breeding habitat and range shifts? Results indicate the majority of species have experienced poleward range shifts over the last 43 years, and birds breeding in all habitat showed trends of poleward shift but only those species breeding in scrub-shrub and grassland environments were different from zero. Additionally, species that are short distance migrants are experiencing significant poleward shifts while Neotropical and permanent residents had shifts that were not different from zero. Our findings do support the general trend expected from climate driven changes (i.e., > 52 % shifting poleward), however, the proportion of species exhibiting equatorial shifts (24 %) or no significant shifts (23 %) illustrates the complex interplay between land cover, climate, species interactions, and other forces that can interact to influence breeding ranges over time. Regardless of the mechanisms driving range shifts, our findings emphasize the need for connecting and expanding habitats for those species experiencing range shifts. This research describes the patterns of breeding birds through central North America and we encourage future research to focus on the mechanisms driving these patterns. 相似文献
102.
Siderophores are structurally diverse, complex natural products that bind metals with extraordinary specificity and affinity. The acquisition of iron is critical for the survival and virulence of many pathogenic microbes and diverse strategies have evolved to synthesize, import and utilize iron. There has been a substantial increase of known siderophore scaffolds isolated and characterized in the past decade and the corresponding biosynthetic gene clusters have provided insight into the varied pathways involved in siderophore biosynthesis, delivery and utilization. Additionally, therapeutic applications of siderophores and related compounds are actively being developed. The study of biosynthetic pathways to natural siderophores augments the understanding of the complex mechanisms of bacterial iron acquisition, and enables a complimentary approach to address virulence through the interruption of siderophore biosynthesis or utilization by targeting the key enzymes to the siderophore pathways. 相似文献
103.
Analysis of 400 scats of the maned wolfChrysocyon brachyurus (Illiger, 1815), collected in Serra da Canastra National Park from November 1998 to January 2000 yielded 1688 occurrences
of 69 identified food items, 49.5% of which were represented by plant items and 50.5% by animal items. According to the frequency
of occurrence analysis, the most important food item categories were wolf’s fruit,Parinari obtusifolia (Chrysobalanaceae), small mammals and birds. When the estimated biomass consumption is considered, the animal items became
more important (56.8%) than plant items (43.2%). Small mammals were consumed more in the dry season, while miscellaneous fruits
and arthropods were consumed mostly in the wet season. Functional response was found only for the category miscellaneous fruits;
however, seasonality analysis revealed that wolf’s fruit and small mammals were consumed according to their availability in
the area. Prey selection was observed for most of the small mammal species, emphasizing a high level of consumption ofNecromys lasiurus, Monodelphis sp. andGracilinanus sp. The finding that most of the diet was composed of fruits and animal items from open areas of the savannah-like Cerrado
led us to suggest that the maned wolf spends most of its time preying in this habitat, indicating a need to establish conservation
policies for the Cerrado. 相似文献
104.
Mounira Ben Farhat Ameny Farhat Wacim Bejar Radhouan Kammoun Kameleddine Bouchaala Amin Fourati Hani Antoun Samir Bejar Hichem Chouayekh 《Archives of microbiology》2009,191(11):815-824
The mineral phosphate solubilizing (MPS) ability of a Serratia marcescens strain, namely CTM 50650, isolated from the phosphate mine of Gafsa, was characterized on a chemically defined medium (NBRIP
broth). Various insoluble inorganic phosphates, including rock phosphate (RP), calcium phosphate (CaHPO4), tri-calcium phosphate (Ca3(PO4)2) and hydroxyapatite were tested as sole sources of phosphate for bacterial growth. Solubilization of these phosphates by
S. marcescens CTM 50650 was very efficient. Indeed, under optimal conditions, the soluble phosphorus (P) concentration it produced reached
967, 500, 595 and 326 mg/l from CaHPO4, Ca3(PO4)2, hydroxyapatite and RP, respectively. Study of the mechanisms involved in the MPS activity of CTM 50650, showed that phosphate
solubilization was concomitant with significant drop in pH. HPLC-analysis of culture supernatants revealed the secretion of
gluconic acid (GA) resulting from direct oxidation pathway of glucose when the CTM 50650 cells were grown on NBRIP containing
glucose as unique carbon source. This was correlated with the simultaneous detection by PCR for the first time in a S. marcescens strain producing GA, of a gene encoding glucose dehydrogenase responsible for GA production, as well as the genes pqqA, B, C and E involved in biosynthesis of its PQQ cofactor. This study is expected to lead to the development of an environmental-friendly
process for fertilizer production considering the capacity of S. marcescens CTM 50650 to achieve yields of P extraction up to 75% from the Gafsa RP. 相似文献
105.
Mercedes Soriano Luis Cistué María P. Vallés Ana M. Castillo 《Plant Cell, Tissue and Organ Culture》2007,91(3):225-234
The aim of this work was to study the effects of colchicine application on chromosome doubling and androgenic response in
anther and microspore culture of different bread wheat genotypes. Colchicine was applied during a mannitol stress pretreatment
or during the first 48 h of culture at concentrations of 0, 150 and 300 mg l−1. When colchicine was applied during stress pretreatment, the percentage of doubling depended on genotype and concentration.
A significant increase in doubling was observed with 300 mg l−1 in the low androgenic responding cv. Caramba. Colchicine incorporation during the first hours of culture improved percentage
of doubling in all genotypes, in both anther and microspore culture. Application of 300 mg l−1 colchicine improved the percentage of doubling in the two low responding genotypes, to 118% of control in DH24033, and 75%
in Caramba in microspore and anther culture, respectively. Concerning the androgenic response, the effect of colchicine on
embryo formation and percentage of green plants depended on the genotype and on the culture method. In cv. Pavon, a 2- and
a 3-fold increase in percentage of embryogenesis and green plants, respectively, were obtained with 300 mg l−1 colchicine in microspore culture. However, no significant differences in these two variables were observed in anther culture.
The number of green doubled haploid (DH) plants reflects the index of success of the procedure. Regardless of the culture
method, when colchicine was incorporated during the first hours of culture, the number of green DH plants increased significantly
in three of four genotypes. These results confirm the usefulness of colchicine application during the first hours of culture
in wheat breeding programs. 相似文献
106.
Decrease or growth of population comes from the interplay of death and birth (and locally, migration). We revive the logistic
model, which was tested and found wanting in early-20th-century studies of aggregate human populations, and apply it instead
to life expectancy (death) and fertility (birth), the key factors totaling population. For death, once an individual has legally
entered society, the logistic portrays the situation crisply. Human life expectancy is reaching the culmination of a two-hundred
year-process that forestalls death until about 80 for men and the mid-80's for women. No breakthroughs in longevity are in
sight unless genetic engineering comes to help. For birth, the logistic covers quantitatively its actual morphology. However,
because we have not been able to model this essential parameter in a predictive way over long periods, we cannot say whether
the future of human population is runaway growth or slow implosion. Thus, we revisit the logistic analysis of aggregate human
numbers. From a niche point of view, resources are the limits to numbers, and access to resources depends on technologies.
The logistic makes clear that for homo faber, the limits to numbers keep shifting. These moving edges may most confound forecasting
the long-run size of humanity. 相似文献
107.
The removal of polycyclic aromatic hydrocarbons by membrane bioreactor (MBR) under aerobic conditions had been studied using naphthalene (NAP) and phenanthrene (PHE) as model compounds. Three MBRs with submerged ultra-filtration hollow fiber membranes were operated applying different operational conditions during 6.5 months. Complete NAP and PHE removal was obtained applying loads of 7 gNAP kgTSS?1 day?1 and 0.5 gPHE kgTSS?1 day?1, while the organic loading rate was adjusted to 0.26 kgCOD kgTSS?1 day?1, with the biomass concentration being 6000 mgTSS L?1, the hydraulic retention time (HRT) 8 h and the solids retention time (SRT) 30 days. Load increases, as well as HRT and SRT reductions, affected the NAP and PHE removals. Biodegradation was found to be the major NAP and PHE removal mechanism. There was no NAP accumulation in the biomass. Low PHE quantities remain sorbed in the biomass and the contribution of the sorption in the removal of this compound was estimated to be less than 0.01 %. The volatilization does not contribute to the PHE removal in MBRs, but the contribution of NAP volatilization can reach up to 0.6 % when HRT of 8 h is applied. 相似文献
108.
Background
Evolution of parasite traits is inextricably linked to their hosts. For instance one common definition of parasite virulence is the reduction in host fitness due to infection. Thus, traits of infection must be viewed in both protagonists and may be under shared genetic and physiological control. We investigated these questions on the oomycete Hyaloperonospora arabidopsis (= parasitica), a natural pathogen of the Brassicaceae Arabidopsis thaliana. 相似文献109.
Signatures of seaway closures and founder dispersal in the phylogeny of a circumglobally distributed seahorse lineage 总被引:1,自引:0,他引:1
Peter R Teske Healy Hamilton Conrad A Matthee Nigel P Barker 《BMC evolutionary biology》2007,7(1):138
Background
The importance of vicariance events on the establishment of phylogeographic patterns in the marine environment is well documented, and generally accepted as an important cause of cladogenesis. Founder dispersal (i.e. long-distance dispersal followed by founder effect speciation) is also frequently invoked as a cause of genetic divergence among lineages, but its role has long been challenged by vicariance biogeographers. Founder dispersal is likely to be common in species that colonize remote habitats by means of rafting (e.g. seahorses), as long-distance dispersal events are likely to be rare and subsequent additional recruitment from the source habitat is unlikely. In the present study, the relative importance of vicariance and founder dispersal as causes of cladogenesis in a circumglobally distributed seahorse lineage was investigated using molecular dating. A phylogeny was reconstructed using sequence data from mitochondrial and nuclear markers, and the well-documented closure of the Central American seaway was used as a primary calibration point to test whether other bifurcations in the phylogeny could also have been the result of vicariance events. The feasibility of three other vicariance events was explored: a) the closure of the Indonesian Seaway, resulting in sister lineages associated with the Indian Ocean and West Pacific, respectively; b) the closure of the Tethyan Seaway, resulting in sister lineages associated with the Indo-Pacific and Atlantic Ocean, respectively, and c) continental break-up during the Mesozoic followed by spreading of the Atlantic Ocean, resulting in pairs of lineages with amphi-Atlantic distribution patterns. 相似文献110.
D. Padilla F. Acosta J. A. García F. Real Jose R. Vivas 《Archives of microbiology》2009,191(3):191-198
Hafnia alvei, a Gram negative bacillus related to the Enterobacteriaceae family, is considered an opportunistic pathogen of several animal species and humans. In this communication, we describe
fimbrial-like structures from different strains of H. alvei that cannot be easily ascribed to any of the previously reported fimbrial types in this species (type I or type III). Polymerase
chain reaction (PCR) and immunofluorescence assays were carried out to study fimbriae and flagella in H. alvei strains isolated from different sources. No correlation between the results obtained by PCR and those obtained by phenotypic
methods were found, and the antibodies used gave cross or different recognition patterns of the surface structures present
in these strains. We report as well that strain and growth temperature influence fimbriation and expression of flagella in
human and animal isolates of H. alvei. This study also indicates that the absence of fimbriae have a significant positive influence on the initial adhesion of
H. alvei to human epithelial cells. 相似文献